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string strAppPoolName = "MyAppPool";
string strUserName = Environment.UserDomainName + "\\" + "myappusername";
addUserAccount(strUserName, strUserPass);
using (ServerManager serverManager = new ServerManager())
{
    //Add application pool
    ApplicationPool appPool = serverManager.ApplicationPools.Add(strAppPoolName);
    appPool.AutoStart = true;
    appPool.ManagedPipelineMode = ManagedPipelineMode.Integrated;
    appPool.ManagedRuntimeVersion = "v4.0";
    appPool.ProcessModel.MaxProcesses = 1;
    //Assign identity to a custom user account
    appPool.ProcessModel.IdentityType = ProcessModelIdentityType.SpecificUser;
    appPool.ProcessModel.UserName = strUserName;
    appPool.ProcessModel.Password = strUserPass;
}

將用戶添加到Active Directory的位置：

public static void addUserAccount(string sUserName, string sPassword)
{
    using (PrincipalContext oPrincipalContext = new PrincipalContext(ContextType.Domain))
    {
        using (UserPrincipal up = new UserPrincipal(oPrincipalContext))
        {
            up.SamAccountName = sUserName;
            up.SetPassword(sPassword);
            up.Enabled = true;
            up.PasswordNeverExpires = true;
            up.Description = "My app's user account";
            up.Save();
        }
    }
}

問題是，當我稍后將該站點和應用程序添加到該應用程序池下的IIS 7時，Web應用程序無法運行，因為它沒有足夠的權限。更重要的是，對于我來說，某些.NET類(例如System.Security.Cryptography)會因意外錯誤代碼而失敗，即使我手動將此新用戶帳戶的讀/寫權限設置為安裝了我的Web應用程序的文件系統文件夾。